Difference Distance Matrix Analysis of the Alpha1-Beta2 Interface

T -> R2	R2 -> R	T -> R
T -> R	R -> R2	T -> R2
Difference Distance maps for the transformations T->R2->R (upper pannel) and T->R->R2 (lower panel). The X-axis reperesents the alpha1 subunit and the Y-axis the beta2 subunit. Points colored red indicate residues that are closer following the transformation and points in blue indicate residues that move farther apart.

The difference distance maps for the transformations T to R and R to R2 are parallel transformations in that residues that move closer in the transformation T to R move even closer in the R to R2 transformation. On the other hand, the sequence T to R2 and R2 to R represent conflicting transformations, since residues that move apart in the T to R2 transformation come together in the R2 to R transformation and residues that move closer in the T to R2 transformation moive apart in the R2 to R transformation.

The difference distance matrices thus suggest that R is likely an intermediate on the pathway from T to R2.